Hypoglycemic effect of soluble polysaccharide and catechins from green tea on inhibiting intestinal transport of glucose
Yeong-Eun Lee
Department of Food Science and Technology, Sejong University, Seoul, Republic of Korea
Search for more papers by this authorSo-Hee Yoo
Department of Food Science and Technology, Sejong University, Seoul, Republic of Korea
Search for more papers by this authorJin-Oh Chung
AMOREPACIFIC R&D Center, 1920, Youngu-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Search for more papers by this authorMi-Young Park
AMOREPACIFIC R&D Center, 1920, Youngu-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Search for more papers by this authorYong-Deog Hong
AMOREPACIFIC R&D Center, 1920, Youngu-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Search for more papers by this authorSi-Hyun Park
Department of Life Science, Gachon University, 1342 Sungnamdaero Sujunggu, Sungnam, Gyeonggido 13120, Republic of Korea
Search for more papers by this authorCorresponding Author
Tae-Sik Park
Department of Life Science, Gachon University, 1342 Sungnamdaero Sujunggu, Sungnam, Gyeonggido 13120, Republic of Korea
Correspondence to: S-M Shim, Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of Korea, E-mail: [email protected] or T-S Park, Department of Life Science, Gachon University, 1342 Sungnamdaero Sujunggu, Sungnam, Gyeonggido 13120 Republic of Korea. E-mail: [email protected]Search for more papers by this authorCorresponding Author
Soon-Mi Shim
Department of Food Science and Technology, Sejong University, Seoul, Republic of Korea
Correspondence to: S-M Shim, Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of Korea, E-mail: [email protected] or T-S Park, Department of Life Science, Gachon University, 1342 Sungnamdaero Sujunggu, Sungnam, Gyeonggido 13120 Republic of Korea. E-mail: [email protected]Search for more papers by this authorYeong-Eun Lee
Department of Food Science and Technology, Sejong University, Seoul, Republic of Korea
Search for more papers by this authorSo-Hee Yoo
Department of Food Science and Technology, Sejong University, Seoul, Republic of Korea
Search for more papers by this authorJin-Oh Chung
AMOREPACIFIC R&D Center, 1920, Youngu-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Search for more papers by this authorMi-Young Park
AMOREPACIFIC R&D Center, 1920, Youngu-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Search for more papers by this authorYong-Deog Hong
AMOREPACIFIC R&D Center, 1920, Youngu-daero, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Search for more papers by this authorSi-Hyun Park
Department of Life Science, Gachon University, 1342 Sungnamdaero Sujunggu, Sungnam, Gyeonggido 13120, Republic of Korea
Search for more papers by this authorCorresponding Author
Tae-Sik Park
Department of Life Science, Gachon University, 1342 Sungnamdaero Sujunggu, Sungnam, Gyeonggido 13120, Republic of Korea
Correspondence to: S-M Shim, Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of Korea, E-mail: [email protected] or T-S Park, Department of Life Science, Gachon University, 1342 Sungnamdaero Sujunggu, Sungnam, Gyeonggido 13120 Republic of Korea. E-mail: [email protected]Search for more papers by this authorCorresponding Author
Soon-Mi Shim
Department of Food Science and Technology, Sejong University, Seoul, Republic of Korea
Correspondence to: S-M Shim, Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of Korea, E-mail: [email protected] or T-S Park, Department of Life Science, Gachon University, 1342 Sungnamdaero Sujunggu, Sungnam, Gyeonggido 13120 Republic of Korea. E-mail: [email protected]Search for more papers by this authorAbstract
BACKGROUND
Water soluble polysaccharide derived from green tea (WSP) is produced as byproducts when catechins were extracted from green tea. Although inhibitory effect of green tea catechins on the glucose transport in small intestine has been studied, the hypoglycemic efficacy of the WSP or its combinational effect has not been studied. In order to investigate hypoglycemic efficacy of the WSP or its combinational effect with green tea extract (GTE), co-consumption of GTE and WSP with wheat starch was investigated using in vitro digestion coupled with Caco-2 cells. The mechanism of the intestinal glucose transport was elucidated throughout the gene expression of the intestinal glucose transporters, which included sodium dependent glucose transporter (SGLT1) and glucose transporter 2 (GLUT2), using quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS
The co-digestion of wheat starch with GTE during the small intestinal phase was the most rapidly digested into reducing sugar (73.96 g L−1) compared to itself (48.44 g L−1), WSP (60.35 g L−1), and GTE + WSP (61.81 g L−1). Intestinal glucose transport was 11.82, 7.59, 4.49, and 2.40% for wheat starch, wheat starch with GTE, WSP, and GTE + WSP, respectively. The highest decreased expression pattern in SGLT1 was observed when cells treated with wheat starch + GTE + WSP (0.66-fold) compared to GTE or WSP treatment.
CONCLUSION
The results suggested that co-consumption of green tea derived products with wheat starch could delay the intestinal absorption of glucose. Results from the current study suggested that GTE and WSP could be the useful supplements of dietary therapy for hyperglycemia to delay glucose absorption. © 2020 Society of Chemical Industry
CONFLICTS OF INTEREST
The authors declare no conflict of interest.
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